DocumentCode :
2055380
Title :
Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic Beads for Virus Detection
Author :
Lien, Kang-Yi ; Wan-Chi Lee ; Lei, Huan-Yao ; Gwo-Bin Lee
Author_Institution :
Inst. of MEMS Eng., Nat. Cheng Kung Univ., Tainan
fYear :
2006
fDate :
18-21 Jan. 2006
Firstpage :
668
Lastpage :
673
Abstract :
The paper presents an integrated automatic micro reverse transcription polymerase chain reaction (RT-PCR) system for molecular diagnosis. The developed system can detect viruses with a high sensitivity and specificity using antibody-conjugated superparamagnetic beads. The target viruses were first captured by conjugated antibodies on the magnetic beads and then attracted by a magnetic field generated by micro-electromagnets. After washing and a purification process, the virus RNA was extracted and transcripted to complementary deoxyribonucleic acid (cDNA), followed by a nucleic acid amplification process using a micro RT-PCR module. With this approach, the virus can be successfully enriched and analyzed. The integrated system performed the whole process automatically utilizing an on-chip integrated microfluidic module. This study performs the successful detection of two different types of viruses, Dengue virus serotype 2 and Enterovirus 71 (EV71), using the developed integrated system. The integrated microsystem can achieve automatic mixing, incubation, purification, transportation, and nucleic acid amplification of the target virus, making it a crucial platform for biological and medical applications
Keywords :
DNA; microfluidics; microorganisms; molecular biophysics; superparamagnetism; Dengue virus serotype 2; EV71; Enterovirus 71; RNA; RT-PCR system; automatic mixing; cDNA; complementary deoxyribonucleic acid; conjugated antibodies; incubation; magnetic fields; micro-electromagnets; microfluidics; molecular diagnosis; nucleic acid amplification; purification; reverse transcription polymerase chain reaction; super-paramagnetic beads; transportation; virus detection; Magnetic analysis; Magnetic fields; Microfluidics; Polymers; Purification; RNA; Sensitivity and specificity; System-on-a-chip; Transportation; Viruses (medical); MEMS; Magnetic bead; Microfluidics; Reverse Transcription Polymerase Chain Reaction; Virus detection;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on
Conference_Location :
Zhuhai
Print_ISBN :
1-4244-0139-9
Electronic_ISBN :
1-4244-0140-2
Type :
conf
DOI :
10.1109/NEMS.2006.334869
Filename :
4135042
Link To Document :
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