Research on Optic Biosensor for Rapid Detection of Hemoglobin

Attempts to develop effective and low cost techniques for blood screening and detection of hemoglobin have become very important. In this work, a home-made biosensor for rapid detection of hemoglobin has been proposed and established on the basis of the reaction of hemoglobin and potassium ferricyanide and the spectral absorption/reflection principle. Using the fast evaporation technique, the reaction reagent can easily form micro crystals and disperse over the reaction membrane uniformly to a certain extent. Then the reaction speed was enhanced. This kind of new type hemoglobin biosensor can be used to detect hemoglobin concentration in 60 seconds at room temperature with a linear range of 5.0~16.0 g/dL, and its correlation coefficient(r) is 0.991.