Title :
Disposable Smart Immunosensing Biochip Using a Novel Electrochemical signaling Method
Author :
Seok Hui Im ; Sin Wook Park ; Jun Hwang Lee ; Hyoung Kil Choi ; Yoon, Hyun C. ; Sang Sik Yang
Author_Institution :
Sch. of Electron. & Comput. Eng., Ajou Univ., Suwon
Abstract :
This paper presents a smart immunosensing biochip using a novel electrochemical method for the portable clinical diagnostics system. The fluid flow of this device can be induced by capillary attraction force only and controlled by a geometrical structure. By this fluid transport mechanism, it does not require external electrical power or control circuit parts. Therefore it is suitable for disposable and portable lab-on-a-chip applications. The fabricated biochip consists of the two poly(dimethyl siloxane) (PDMS) layers and a glass substrate for immunoelectrodes. The size of the biochip is 25times20times6 mm3. The signal amplification strategy applied in this biochip is based on the back-filling immobilization of biocatalytic enzyme at immunoelectrodes, circumventing the use of enzyme-labeled antibody. For the immunosensing surface construction, a poly(amidoamine) G4-dendrimer was employed as a matrix for ligand functionalization. As a model biorecognition reaction, the dinitrophenyl (DNP) antigen-functionalized electrode was fabricated and an anti-DNP antibody was used. Glucose oxidase (GOX) was chosen to amplify electrochemical signal by enzymatic catalysis. The non-labeled native antibody was biospecfically bound to the immobilized ligand, and the activated enzyme (periodate-treated GOX) reacted and back-filled the remaining surface amine group on the dendrimer layer
Keywords :
bioMEMS; biosensors; capillarity; electrochemical electrodes; electrochemical sensors; electrochemistry; enzymes; microfluidics; patient diagnosis; polymers; 20 mm; 25 mm; 6 mm; DNP antigen functionalized electrode; PDMS layers; activated enzyme; antiDNP antibody; biocatalytic enzyme back filling immobilization; biorecognition reaction; capillary attraction force; dendrimer layer; device fluid flow; dinitrophenyl; electrochemical signal amplification; electrochemical signaling method; enzymatic catalysis; fluid transport mechanism; glass substrate; glucose oxidase; immunoelectrodes; immunosensing surface construction; ligand functionalization; periodate-treated GOX; poly-dimethylsiloxane; polyamidoamine G4-dendrimer; portable clinical diagnostics; portable lab-on-a-chip applications; smart immunosensing biochip; Biochemistry; Circuits; Electrodes; Fluid flow; Fluid flow control; Force control; Glass; Immune system; Lab-on-a-chip; Sugar; PDMS; back-filling; capillary attraction force; glucose oxidase; immunosensing;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on
Conference_Location :
Zhuhai
Print_ISBN :
1-4244-0139-9
Electronic_ISBN :
1-4244-0140-2
DOI :
10.1109/NEMS.2006.334673