Superimposed AC- and DC electric field guided deposition of a single DNA molecule along a microfabricated gap

A single DNA molecule was deposited along a two-electrode microfabricated gap, using the Composite Electric-field Guided Assembly (CEGA) method. The suggested nano-assembly technique based on a superimposed ac- and dc electric field enabled attraction of a single DNA molecule out of an extremely diluted aqueous solution (∼10 aM), subsequent stretching and deposition of the molecule along a gap formed by a pair of electrodes. The stretching of the single DNA is attributed to an electrohydrodynamic flow generated by the electric field.