Experimental Procedures for Fluorescent Lifetime Measurements with Customized Embedded DSP Systems and Adaptive Algorithms

Author

Arul, Ann D. ; Snyder, Andy ; Kelley, Redmond ; Meier, John ; Hicks, Tyler ; Yeary, Mark ; McCann, Patrick ; Roller, Chad

Author_Institution

Univ. of Oklahoma, Norman

fYear

2007

fDate

20-22 April 2007

Firstpage

124

Lastpage

128

Abstract

This paper describes a method of utilizing UV lasers, detectors, and a high-speed digital signal processor (DSP)-based digitizer to measure the fluorescence lifetime of fluorophores. The aim of the design is to create a low cost compact device which guarantees ease of use while not compromising performance. An ultrafast photomultiplier tube coupled with an excitation light filter is used to characterize accurately the fluorescence emission from the sample. The results obtained from several material specimens are presented.

Keywords

analogue-digital conversion; fluorescence spectroscopy; optical couplers; optical filters; organic compounds; photodetectors; photomultipliers; radiative lifetimes; spectrochemical analysis; UV lasers; adaptive algorithms; detectors; excitation light filter; fluorescence lifetime; fluorophores; high-speed digital signal processor-based digitizer; ultrafast photomultiplier tube; Adaptive algorithm; Costs; Digital signal processing; Digital signal processors; Filters; Fluorescence; Laser excitation; Lifetime estimation; Optical coupling; Photomultipliers; Fluorescence; biodetection; high-speed undersampling; laser applications; lifetime spectroscopy; swept delay;

fLanguage

English

Publisher

ieee

Conference_Titel

Region 5 Technical Conference, 2007 IEEE

Conference_Location

Fayetteville, AR

Print_ISBN

978-1-4244-1280-8

Electronic_ISBN

978-1-4244-1280-8

Type

conf

DOI

10.1109/TPSD.2007.4380365

Filename

4380365