DocumentCode :
2067171
Title :
Manipulation and observation of binding of molecules driven by motor proteins
Author :
Fujimoto, Kenji ; Kitamura, Masayuki ; Shintaku, Hirofumi ; Kotera, Hidetoshi ; Yokokawa, Ryuji ; Yokokawa, M.
Author_Institution :
Dept. Micro Eng., Kyoto Univ., Kyoto, Japan
fYear :
2012
fDate :
4-7 Nov. 2012
Firstpage :
66
Lastpage :
69
Abstract :
We developed a system to visualize molecular bindings as colocalization of two Q-dots carried by motor proteins on a microtubule (MT) array with predefined polarities in nanotracks. The MT array was prepared utilizing gliding of MTs in micro/nano structures and a dissociation method of retrogressing MTs. Pairs of molecules for cargo transport was designed and evaluated experimentally. One of molecular systems, GSH-Q-dot525-dynein (GSH-Q525-D) and GST-Q-dot655-kinesin (GST-Q655-K), resulted in colocalizations by the GST-GSH binding. The other system, avidin-Q525-D (avi-Q525-D) and biotin-Q655-K (bio-Q655-K), also provided colocalization of Q-dots by specific bindings of avidin-biotin. Analysis of run length (RL) and velocity showed molecular transport without degrading motility of motor proteins. Quantitative analysis proved that colocalizations were achieved by the designed molecules carried by motors. The results show that a prototype of synthetic molecular transport driven by motor proteins was established. The reconstructed molecular system should be the basis of further miniaturization of on-chip analysis systems by directly manipulating countable number of molecules.
Keywords :
biotransport; dissociation; molecular biophysics; molecular configurations; proteins; quantum dots; GSH-Q-dot525-dynein; GST-GSH binding; GST-Q-dot655-kinesin; avidin-Q525-D; biotin-Q655-K; cargo transport; dissociation method; microtubule array; microtubule gliding; molecular binding; molecular systems; motor proteins; nanotrack polarity; on-chip analysis systems; quantitative analysis; quantum dots; synthetic molecular transport; Dynein; Key words; Kinesin; Microtubule; Molecular reaction; Motor proteins; Nanotransport;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Molecular Medicine and Engineering (NANOMED), 2012 IEEE 6th International Conference on
Conference_Location :
Bangkok
ISSN :
2159-6964
Print_ISBN :
978-1-4673-5101-0
Type :
conf
DOI :
10.1109/NANOMED.2012.6509120
Filename :
6509120
Link To Document :
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