Cytotoxicity effects of lipophilic extracts of Thai herbs in human breast cancer cells and Human Epidermoid Carcinoma cells

This study was to evaluate the cytotoxic activity of lipophilic extracts of Thai herbs (Harrisonia perforata, Pongamia Pinnata, Azadirachta indica) against from Human Breast Cancer (MCF-7) cells, Human Epidermoid Carcinoma (KB-31) cells and from Monkey kidney (Vero) cells were tested by MTT assay against Vero, KB-31 and MCF-7 cells line. Apoptotic induction was tested in the MCF-7 and used Vero cells as control was analyzed by electrophoresis. All extracts did not induce cytotoxicity against Vero cells. A. indica leaves showed the highest cytotoxicity effect against MCF-7 cells (IC₅₀ = 296.49 μg/ml) and KB-31 cells (IC₅₀ 199.49 μg/ml), whereas H.perforata stem bark showed the highest cytotoxicity effect against KB-31 cells (IC₅₀= 191.06μ/ml). Leaves of H.perforata and P.pinnata had not produced cytotoxicity against three cell lines.