Title :
Single-cell detection and classification in phase contrast images
Author :
Athelogou, M. ; Eblenkamp, M. ; Schmidt, G. ; Novotny, F. ; Wintermantel, E. ; Binnig, G.
Author_Institution :
Definiens AG, Munich, Germany
Abstract :
This paper presents a method for the fully automated segmentation, classification and quantification of the morphology of individual cells in phase-contrast images. We utilize this method to calculate automatically the confluence of the corresponding cell culture and to quantify the degree of toxic damage of each individual cell after phenol incubation. These results are compared to standard cytotoxicity assays.
Keywords :
cellular biophysics; image classification; image segmentation; medical image processing; cell classification; cell culture; cell morphology; cytotoxicity assay; fully automated segmentation; phase contrast image; single cell detection; Biology; Biomedical imaging; Image segmentation; Phase measurement;
Conference_Titel :
Information Technology and Applications in Biomedicine (ITAB), 2010 10th IEEE International Conference on
Conference_Location :
Corfu
Print_ISBN :
978-1-4244-6559-0
DOI :
10.1109/ITAB.2010.5687789
