Title :
Towards ultrahigh throughput microinjection: MEMS-based massively-parallelized mechanoporation
Author :
Yanyan Zhang ; Ballas, C.B. ; Rao, M. Purnachandra
Author_Institution :
Dept. of Mech. Eng., Univ. of California, Riverside, Riverside, CA, USA
fDate :
Aug. 28 2012-Sept. 1 2012
Abstract :
We describe a massively-parallelized, MEMS-based device concept for passively delivering exogeneous molecules into living cells via mechanical membrane penetration, i.e., mechanoporation. Details regarding device design and fabrication are discussed, as are results from preliminary live cell studies focused on device validation at the proof-of-concept level. These efforts represent key steps towards our long-term goal of developing instrumentation capable of ultrahigh throughput (UHT) cellular manipulation via active microinjection.
Keywords :
bioMEMS; biological techniques; biomechanics; biomembranes; cellular biophysics; macromolecules; molecular biophysics; MEMS- based device; MEMS-based massively-parallelized mechanoporation; device design; living cells; mechanical membrane penetration; passively delivering exogeneous molecules; proof-of-concept level; ultrahigh throughput cellular manipulation; ultrahigh throughput microinjection; Cells (biology); Educational institutions; Fabrication; Instruments; Microinjection; Throughput; USA Councils; Equipment Design; Flow Cytometry; Humans; K562 Cells; Micro-Electrical-Mechanical Systems; Microinjections; Microscopy, Electron, Scanning;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4244-4119-8
Electronic_ISBN :
1557-170X
DOI :
10.1109/EMBC.2012.6346001
