Towards ultrahigh throughput microinjection: MEMS-based massively-parallelized mechanoporation

Author

Yanyan Zhang ; Ballas, C.B. ; Rao, M. Purnachandra

Author_Institution

Dept. of Mech. Eng., Univ. of California, Riverside, Riverside, CA, USA

fYear

2012

fDate

Aug. 28 2012-Sept. 1 2012

Firstpage

594

Lastpage

597

Abstract

We describe a massively-parallelized, MEMS-based device concept for passively delivering exogeneous molecules into living cells via mechanical membrane penetration, i.e., mechanoporation. Details regarding device design and fabrication are discussed, as are results from preliminary live cell studies focused on device validation at the proof-of-concept level. These efforts represent key steps towards our long-term goal of developing instrumentation capable of ultrahigh throughput (UHT) cellular manipulation via active microinjection.

Keywords

bioMEMS; biological techniques; biomechanics; biomembranes; cellular biophysics; macromolecules; molecular biophysics; MEMS- based device; MEMS-based massively-parallelized mechanoporation; device design; living cells; mechanical membrane penetration; passively delivering exogeneous molecules; proof-of-concept level; ultrahigh throughput cellular manipulation; ultrahigh throughput microinjection; Cells (biology); Educational institutions; Fabrication; Instruments; Microinjection; Throughput; USA Councils; Equipment Design; Flow Cytometry; Humans; K562 Cells; Micro-Electrical-Mechanical Systems; Microinjections; Microscopy, Electron, Scanning;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE

Conference_Location

San Diego, CA

ISSN

1557-170X

Print_ISBN

978-1-4244-4119-8

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/EMBC.2012.6346001

Filename

6346001