Study on the apoptosis effect induced by isothiocyanates in broccoli on human gastric adenoma cells (SGC-7901)

To investigate the pro-apoptosis effect of isothiocyanates (ITCS) on human gastric adenoma cells SGC-7901, and its mechanism. SGC-7901 cells were treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test ROS levels, intracellular mitochondrial transmembrane potential(Deltapsim), and hypodiploid apoptosis peak in SGC-7901 cells. ITCS obviously inhibited proliferation of SGC-7901 cells. When treated with 0, 15, 30, 60, 120, 240 mug /mL of ITCS for 24 h, ROS levels were (1.6plusmn0.5)%, (2.0plusmn0.3)%, (5.5+0.4)%, (25.8plusmn1.4)%, (83.7plusmn1.2)% and (97.4+4.2)%, respectively; and Deltapsim were (98.6plusmn4.3)%, (98.4+4.8)%, (95.7+5.4)%, (92.6+4.0)%, (74.0+5.6)% and (63.7plusmn4.0)%, respectively; when treated with 0, 60, 120, 240 mug /mL of ITCS for 48 h, cell apoptotic rates were (4.3plusmn1.6)%, (9.1plusmn3.8)%, (20.1+4.2)% and (55.4+4.9)%, respectively. ITCS generates ROS in gastric cancer SGC-7901 cells, which causes mitochondrial membrane permeabilization and Deltapsim decrease, therefore, leads to apoptosis of SGC-7901 cells.