An Optical Manner for Identification Tumor Cells

According to the morphologic character of cells, a simple way based on the circular aperture diffraction is put forward in the paper. Cells in different states, such as normal living cells, apoptosis cells, necrosis cells, can be differentiated easily and the quantities and the quantities ratio of cells in different states can be got in this way. The diameter of sample cell can be measured accurately by the circular aperture diffraction combined with CCD. The frame diagram of measuring the size of cells based on the circular diffraction and CCD is presented, as well as the Principle of method in the paper. Using the method presented in the paper, the apoptosis and necrosis situations of leukaemia tumour cell HL60 were measured. The experiment results showed that the results of quantities and the quantities ratios of HL60 cells in different states such as apoptosis and necrosis measured by the method presented in the paper were consistent with the results measured by the other accepted method.