Title :
On-chip electroporation: Characterization, modeling and experimental results
Author :
Ghafar-Zadeh, Ebrahim ; Gholamzadeh, B. ; Awwad, F. ; Sawan, Mohamad
fDate :
Aug. 28 2012-Sept. 1 2012
Abstract :
In this paper, we describe an on-chip electroporation (EP) method for high precision nano-injection of bio-molecules into single cells. EP is an electrical stimulation method to create nano-pores on the cell plasma membrane. Herein, we first put forward the computational models of the cultured cells microelectrodes. We thereafter discuss practical considerations by demonstrating the preliminary experimental results. The mouse fibroblast cells are cultured above electrodes while experiencing a low frequency (10 Hz) electrical field (EF) in the presence of propidium iodide (PI).
Keywords :
bioelectric phenomena; biological techniques; biomembranes; cellular biophysics; microelectrodes; biomolecules; cell plasma membrane; electrical stimulation method; high precision nanoinjection; microelectrode; mouse fibroblast cell; nanopores; on chip electroporation; propidium iodide; Arrays; Cells (biology); Electrodes; Fibroblasts; Gold; Microelectronics; Microfluidics; Electric Stimulation; Electrodes; Electroporation; Models, Theoretical;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4244-4119-8
Electronic_ISBN :
1557-170X
DOI :
10.1109/EMBC.2012.6346492
