In vitro recellularization of aorta scaffolds prepared by sonication treatment

Sonication treatment is used in the preparation of bioscaffolds that was able to support repopulation of vascular smooth muscle cells (VSMCs) upon cell-seeding. The aim of this study is to investigate the ability of sonicatedly decellularized tissue to repopulate VSMCs after 6 days of cell-seeding. In this study, sample of aorta tissues are decellularized by sonication treatment in 0.1% and 2% sodium dodecyl sulfate (SDS) detergent for 10 hours. It was followed by washing process with PBS solution for 5 days. Decellularized aorta tissues are then cell seeded with VSMCs by static seeding in 96-well plate containing Dulbecco´s Modified Eagle Medium (DMEM) at 37°C. The infiltrations of VSMCs onto decellularized tissues are evaluated by comparison of Hematoxylin-Eosin (H-E) staining at 0 and 6 days of cell-seeding. The histological results of cell-seeding showed that VSMCs are able to infiltrate onto the decellularized tissues. From the results, sonicatedly decellularized tissue treated in 0.1% and 2% SDS, seeded with VSMCs showed infiltration depth of 0.43 mm and 0.35 mm, respectively. Hence, a sonicated decellularized tissue treated with 0.1% and 2% SDS was shown to support the repopulation of VSMCs.