Glucosinolates in ten cultivars of Chinese kale (Brassica alboglabra L.H Bailey) seperation and identification by HPLC-PAD-ESI/MS

A little information is available on the kinds and contents of glucosinolate (GS) in Chinese kale (Brassica alboglabra L.H.Bailey). The objectives were to separate and identify the GSs in Chinese kale by HPLC-PAD-ESI/MS, and determine the variety of GSs in ten cultivars widely grown in China. Eleven GSs were separated and identified in bolting stems of Chinese kale among ten cultivars, including seven aliphatic GSs and four indolyl GSs. Total GS content in bolting stem varied significantly and ranged from 5.21 to 11.92 μmol g^-1 DW among ten cultivars of Chinese kale. The aliphatic GSs were predominant, representing 81.63% of the total glucosinolate content on average. The presence of high concentration of sinigrin, glucoraphanin, glucoiberin, and glucobrassicin in bolting stems warrant further research into their potential use to enhance the level of these important phytochemicals in Chinese kale.