Title :
A Novel Method to Enhance Sensitivity in HPLC Detection
Author :
Wei, Yuxia ; Xiao, Shengyuan ; Mu, Xiaoling ; Zhu, Yong ; Hu, Gaofei ; Deng, Yulin ; Yang, Xinlin
Author_Institution :
Beijing Inst. of Technol., Beijing
Abstract :
The peak compression effect induced by velocity difference has been theoretically and experimentally established. L-tryptophan and 2-nitrophenol were used as model compounds. The peak compression of L-tryptophan and 2-nitrophenol was obtained by the difference of its migration velocity along the column induced by the adjustment of composition of mobile phase. A compression of analytes peak and reduced tailing was observed. Under optimum compression condition, 17-fold and 5.9-fold improvement in the detection sensitivity was obtained compared to elution under non-compressed conditions for L-tryptophan and 2-nitrophenol, respectively.
Keywords :
chromatography; molecular biophysics; organic compounds; 2-nitrophenol; HPLC detection; L-tryptophan; migration velocity; peak compression effect; velocity difference; Detectors; Hardware; Phase detection; Shape; Signal analysis; Signal to noise ratio; Solvents; Switches; HPLC; detection; sensitivity;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4382052
