Immobilization of Trypsin onto Artificial Membrane for the Possible Application in the Digestion Reactor of Proteins

This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400 mum I.D.times690 mum O.D.times5 cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1 mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 mul/min was obviously larger than that at flow rate of 6 mul/min. The bioactivity of immobilized trypsin was at least 69.3% in 24 h.