Title :
The Investigation of Dynamically Immobilized MAO on JAM Using Biologically Functionalized Chromatography
Author :
Liu, Yueying ; Dai, Rongji ; Qu, Feng ; Meng, Weiwei ; Deng, Yulin
Author_Institution :
Beijing Inst. of Technol., Beijing
Abstract :
In this paper, the retention times of serotonin (5-HT) were investigated on IAM (immobilized artificial membrane) column and IAM-MAO column which MAO was inhibited by clorgyline at various concentrations. The retention time of 5-HT on IAM column was shorter than that on IAM-MAO column. With the increasing of inhibitor concentrations, the retention time of 5-HT on IAM-MAO column became shorter and shorter. The results demonstrated that the MAO was successfully immobilized on IAM. The dynamical immobilized MAO retained its bioactivity. The establishment of artificial cell could better simulate the interaction between 5-HT and MAO in organism. Thus this study proved that BioFC (biologically functionalized chromatography) might become a new concept and method to simulate cell (artificial cell) and a possible tool for the drug screening in the future.
Keywords :
biochemistry; biomembranes; cellular biophysics; chromatography; drugs; enzymes; molecular biophysics; 5-HT; BioFC; IAM column; IAM-MAO column; artificial cell simulation; bioactivity; biologically functionalized chromatography; clorgyline; drug screening; dynamically immobilized MAO; immobilized artificial membrane; inhibitor concentration; momoamine oxidase; retention time; serotonin; Biological processes; Biological system modeling; Biology; Biomembranes; Cells (biology); Humans; Inhibitors; Organisms; Positron emission tomography; Proteins; 5-HT; MAO; clorgyline; dynamical immobilization;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4382055
