Trierpenic acids isolated from Betula birch inhibits angiogenesis in vitro and in vivo

Some trierpenic acids as a effective part for anti-tumor were extracted from Betula birch, HPLC and UV analysis methods were used to optimize extraction artwork and control the total trierpenic acids in the extraction for quantity control especially; In this study, we investigate the inhibitory effects of Trierpenic acids isolated from Betula birch TABB on angiogenesis. The in vitro anti-angiogenic effect of Trierpenic acids isolated from Betula was tested on models of angiogenesis : proliferation , migration of human umbilical vascular endothelial (ECV304) cell; The anti-angiogenic effect in vivo was detected in morphological development on model of chick chorioallantoic membrane. Cox-2mRNA expression was detected by RT-PCR. We found TABB inhibited the growth of human umbilical vascular endothelial (ECV304) cell line in a concentration-dependent manner (1-100 mug/ml).The migration of ECV304 was obviously inhibited by TABB. In CAM assay, TABB could significant inhibited the angiogenesis at the administered dosages (10-30 mug/CAM). And significantly inhibit cox-2 mRNA expression. TABB was shown to inhibit angiogenesis in vitro and in vivo, which may be the mechanism of the treatment of anti-tumor effect.