The pulse exciation of UV LED source for fluorescence detection

Fluorescence is widely used to detect the biochemical effect and some substance containing certain dye. In general, the formation of fluorescent reaction is that an organism or dye, excited by UV light (200-405 nm), emits a specific frequency of light; the light is usually a visible or near infrared light (405-900 nm) However, the object excited by continue UV light cannot provide the periodic response information and clear images for fluorescence analysis, and the fluorescence intensity of organism will decay with the time exposed to UV light. In this study, we use a tunable pulse control module to perform the fluorescence test of security organic dye, BL-ORT. When the BL-ORT is excited by the 30 ms/cycle pulsed UV-LED light, the most complete fluorescence datum can be obtained. Furthermore, the power consumption of pulsed exciting source can be down to 50 percent.