Single molecule tracking of P-glycoprotein in live cells reveals dynamic heterogeneity

Author

Cheney, P.P. ; Stachler, M.D. ; Knowles, M.K.

Author_Institution

Dept. of Chem. & Biochem., Univ. of Denver, Denver, CO, USA

fYear

2012

fDate

Aug. 28 2012-Sept. 1 2012

Firstpage

3159

Lastpage

3162

Abstract

P-glycoprotein transports chemotherapy drugs from the plasma membrane and allows cancer cells to survive treatment. We transiently transfected PGP labeled with enhanced green fluorescent protein (PGP-EGFP) into MES-SA cells and used single molecule tracking techniques to characterize the dynamics on the surface of live cells. PGP exhibits freely diffusive behavior at short times and is confined at long times with a transition to anomalous diffusion at 0.7 s.

Keywords

biodiffusion; biological techniques; biomembrane transport; cancer; drugs; fluorescence spectroscopy; molecular biophysics; proteins; EGFP label; MES-SA cells; P-glycoprotein single molecule tracking; PGP transfection; PGP-EGFP; anomalous diffusion; cancer cells; chemotherapy drug transport; dynamic heterogeneity; enhanced green fluorescent protein; freely diffusive behavior; live cell surface dynamics; live cells; plasma membrane; Biological system modeling; Electronic mail; Fluorescence; Image segmentation; Tracking; Cell Line; Green Fluorescent Proteins; Humans; Microscopy, Confocal; Microscopy, Fluorescence; P-Glycoprotein;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE

Conference_Location

San Diego, CA

ISSN

1557-170X

Print_ISBN

978-1-4244-4119-8

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/EMBC.2012.6346635

Filename

6346635