Studies on the Isolation of Lipase-Producing Strain and Immobilized Condition

Author

Cai Lu ; Ji Xiang

Author_Institution

Inst. of Bioeng. & Technol., Inner Mongolia Univ. of Sci. & Technol., Baotou, China

fYear

2010

fDate

28-31 March 2010

Firstpage

1

Lastpage

3

Abstract

8 strains producing lipase were isolated from soil with rich oil. Among them strain BD3 producing lipase was more powerful. Strain BD3 was identified as Pseudomonas sp according to its morphology, physiological, biochemical properties. We utilized PVA(polyvinyl alcohol) and Boric Acid as the vector of immobilized cells of Strain BD3, and have studied transesterification at the same time. We found that catalytic efficiency of immobilized cells which was made of lower concentration PVA is higher than that of the high concentration PVA obviously. The activity of immobilized cell is lower than that of non-immobilized one. Immobilized cells maintained activity after many batches.

Keywords

biochemistry; biofuel; catalysis; enzymes; inorganic compounds; microorganisms; oils; polymers; soil; PVA; Pseudomonas sp; boric acid; catalytic efficiency; immobilized cells; lipase producing strain isolation; polyvinyl alcohol; rich oil; soil; strain BD3 producing lipase; transesterification; Biochemistry; Biomedical engineering; Capacitive sensors; Fungi; Isolation technology; Morphology; Optical microscopy; Petroleum; Soil; Strain measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Power and Energy Engineering Conference (APPEEC), 2010 Asia-Pacific

Conference_Location

Chengdu

Print_ISBN

978-1-4244-4812-8

Electronic_ISBN

978-1-4244-4813-5

Type

conf

DOI

10.1109/APPEEC.2010.5448487

Filename

5448487