Title :
Lensfree holographic cytometry using plasmonic nanoparticles
Author :
Qingshan Wei ; McLeod, Euan ; Hangfei Qi ; Zhe Wan ; Ren Sun ; Ozcan, Aydogan
Author_Institution :
Electr. Eng. Dept., Univ. of California, Los Angeles, Los Angeles, CA, USA
Abstract :
Cellular contrast was demonstrated for the first time in lensfree on-chip holography by employing antibody-conjugated gold and silver nanoparticles (NPs) as contrast agents. Cytometric differentiation of CD4+ and CD8+ lymphocytes was achieved, after NP labeling, using machine learning algorithms.
Keywords :
CMOS image sensors; blood; cellular biophysics; gold; holography; learning (artificial intelligence); molecular biophysics; nanobiotechnology; nanoparticles; plasmonics; proteins; silver; CD4 lymphocytes; CD8 lymphocytes; NP labeling; antibody-conjugated gold; cellular contrast; contrast agents; cytometric differentiation; lens free on-chip holography cytometry; machine learning algorithms; plasmonic nanoparticles; silver nanoparticles; Absorption; Gold; Holography; Labeling; Nanoparticles; Plasmons; System-on-chip;
Conference_Titel :
Photonics Conference (IPC), 2013 IEEE
Conference_Location :
Bellevue, WA
Print_ISBN :
978-1-4577-1506-8
DOI :
10.1109/IPCon.2013.6656337
