Author_Institution :
Center of Res. & Dev. on Life Sci. & Environ. Sci., Harbin Univ. of Commerce, Harbin, China
Abstract :
The aim of this paper is to investigate the pro-apoptosis effect of isothiocyanates (ITCS) on human gastric adenoma cells SGC-7901 and its mechanism. SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim), and hypodiploid apoptosis peak in both SGC-7901. ITCS obviously inhibited proliferation of SGC-7901 cells. When treated with 0, 15, 30, 60, 120, 240 mug /mL of ITCS for 24 h, intracellular ROS levels of SGC-7901 were (1.6plusmn0.5)%, (2.0plusmn0.3)%, (5.5plusmn0.4)%, (25.8plusmn1.4)%, (83.7plusmn1.2)% and (97.4plusmn4.2)%, respectively; and Deltapsim were (98.6plusmn4.3)%, (98.4plusmn4.8)%, (95.7plusmn5.4)%, (92.6plusmn4.0)%, (74.0plusmn5.6)% and (63.7plusmn4.0)%, respectively; when treated with 0, 60, 120, 240 mug /mL of ITCS for 48 h, cell apoptotic rates of SGC-7901 were (4.3plusmn1.6)%, (9.1plusmn3.8)%, (20.1plusmn4.2)% and (55.4plusmn4.9)%, respectively. ITCS generates ROS in both SGC-7901, which causes mitochondrial membrane permeabilization and Deltapsim decrease, therefore, leads to apoptosis.
Keywords :
biochemistry; bioelectric potentials; biological organs; biomedical measurement; biomembrane transport; cancer; permeability; tumours; MTT assay; apoptosis effect; broccoli; cancer; cell proliferation; flow cytometry; human gastric adenoma cell; hypodiploid apoptosis peak; intracellular mitochondrial transmembrane potential; isothiocyanate; mitochondrial membrane permeabilization; reactive oxygen species level; time 24 h; time 48 h; Biomembranes; Business; Cancer; Cells (biology); Drugs; Fluorescence; Humans; Seminars; Testing; Wavelength measurement;
