Fast cell immobilization by using non-immunological method for cell based biosensor

This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5×10⁶, 1.0×10⁶ or 2.0×10⁶ cells/ml in micro wells with 10μm depth and 25μm, 50μm and 100μm size respectively was performed. Different tendencies of cell adhesion between 0.5×10⁶ cells/ml and 1.0×10⁶ cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.