• DocumentCode
    2122632
  • Title

    Phage Langmuir-Blodgett films for biosensing applications

  • Author

    Olsen, Eric ; Guntupalli, Rajesh ; Sorkulova, Iryna ; Long, Robert ; Neely, William ; Vodyanoy, Vitaly

  • Author_Institution
    Clinical Res. Lab., Keesler AFB, MS, USA
  • fYear
    2010
  • fDate
    1-4 Nov. 2010
  • Firstpage
    401
  • Lastpage
    406
  • Abstract
    The microstructure and functionality of methicillin-resistant Staphylococcus aureus (MRSA) biosensors prepared from novel Langmuir-Blodgett monolayers of lytic bacteriophage were characterized using scanning imaging ellipsometry (SIE) and scanning electron microscopy (SEM). SEM revealed uniform distribution of phage monolayers immobilized to biosensor substrates. SIE indicated monolayers were 49.8 ± 18.3 nm thick on average. SIE charged couple imaging analysis of biosensors yielded an average intensity of 159±7 and 194±13 for 108 and 109 CFU/ml MRSA concentrations, respectively.
  • Keywords
    Langmuir-Blodgett films; biosensors; ellipsometry; microorganisms; molecular biophysics; monolayers; scanning electron microscopy; Langmuir-Blodgett films; SEM; SIE; biosensors; functionality; lytic bacteriophage; methicillin-resistant Staphylococcus aureus; microstructure; scanning electron microscopy; scanning imaging ellipsometry; size 49.8 nm;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Sensors, 2010 IEEE
  • Conference_Location
    Kona, HI
  • ISSN
    1930-0395
  • Print_ISBN
    978-1-4244-8170-5
  • Electronic_ISBN
    1930-0395
  • Type

    conf

  • DOI
    10.1109/ICSENS.2010.5690208
  • Filename
    5690208
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=2122632