The Effect of Sargassum Fusiforme Polysaccharides on the Functioning of Erythrocyte Membrane of S180 Mice by Affecting Ca2+ Channels

Abstract-To analyze the effect of Sargassum fusiforme polysaccharides (SFPS) on the functioning of erythrocyte membrane of S180 mice by affecting Ca²⁺ channels. A tumor mouse model was developed, and the mice were treated with high, medium, and low doses of SFPS. Changes in [Ca²⁺] in the erythrocytes in the tumor-bearing mice were measured using laser confocal scanning; changes in the content of sialic acid and the acitivities of ATPase were meausred using relevant test kits; changes in the level of erythrocyte membrane potential were analyzed using FCM; and changes in the complex mobility of erythrocytes were measured using HPCE. SFPS lowered the intracelluar [Ca²⁺] of erythrocytes in tumor-bearing mice, increased the content of sialic acid on surface of erythrocyte membrane, increased the acitivities of Na⁺,K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase, and raised the level of erythrocyte membrane potential and the complex mobility of erythrocytes in electrophoresis. By affecting the Ca²⁺ channels, SFPS can increase the content of sialic acid on the surface of erythrocyte membrane. The sialic acid combines with Ca²⁺ to increase the activities of Na⁺,K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase, resulting in a rise in the membrane potential of the erythrocytes and an increase in the complex mobility of the erythrocytes in electrophoresis, thus restoring many physiological and biochemical functions of the erythrocyte membrane. This may be one of the anti-tumor mechanisms of SFPS.