Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling

Author

Chen, Yi ; Xu, Zhida ; Liu, Logan ; Coppe, Jean-Philippe

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA

fYear

2010

fDate

1-4 Nov. 2010

Firstpage

2295

Lastpage

2298

Abstract

Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technol ogy will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.

Keywords

biosensors; enzymes; etching; molecular biophysics; molecular configurations; nanosensors; passivation; proteomics; surface enhanced Raman scattering; Rhodamin6G; biological nanosensors; crude peptides; direct proteomic profiling; environment monitoring; etching-passivation process; highly sensitive surface-enhanced Raman nanoprobing; kinase-enzyme activity screening array device; mutation; nanocone structures; nanosensing probes; nanostructured SERS substrate; peptide probe sequences; single amino acid phosphosphorylation; surface enhanced Raman spectroscopy; surface-conjugated peptides;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2010 IEEE

Conference_Location

Kona, HI

ISSN

1930-0395

Print_ISBN

978-1-4244-8170-5

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2010.5690802

Filename

5690802