pH sensor demonstrating a layout programmable squeeze pumped microfluidic platform

We demonstrate a pH sensor utilizing a layout programmed squeeze-pumped microfluidic platform. The device is fabricated via direct polydimethylsiloxane (PDMS) micromilling and utilizes an external single-actuator squeeze pump for simultaneous pumping of multiple fluids at different layout-determined flow volumes from 300-9000pL/step. The pumping system, akin to the development of Polaroid instant film, provides an inexpensive means for operating disposable microfluidic devices in the field without external power supplies, valves, or fluidic interface ports. The pH sensor demonstrates functionality of this low-cost, disposable, lab-on-a chip microfluidic detection system.