Direct nuclear delivery of DNA macromolecules using the photothermal nanoblade

Author

Ting-Hsiang Wu ; Yi-Chien Wu ; Sagullo, Enrico ; Teitell, Michael A. ; Chiou, Pei-Yu Eric

Author_Institution

Univ. of California, Los Angeles, Los Angeles, CA, USA

fYear

2013

fDate

18-22 Aug. 2013

Firstpage

77

Lastpage

78

Abstract

We demonstrated direct nuclear delivery of macromolecules into live mammalian cells using the photothermal nanoblade. Pulsed laser triggered cavitation bubbles on a titanium thin film coated micropipette tip was utilized to puncture both the cell plasma and nuclear membranes followed by pressure controlled delivery of cargo into the nucleus. High plasmid expression (79-100%) in different cell types with maintained cell viability was achieved.

Keywords

DNA; biomembranes; blades; bubbles; cavitation; cellular biophysics; drug delivery systems; drugs; gene therapy; laser applications in medicine; metallic thin films; molecular biophysics; nanomedicine; photothermal effects; titanium; DNA macromolecules; Ti; cell plasma; cell types; cell viability; direct nuclear delivery; high-plasmid expression; live mammalian cells; nuclear membranes; photothermal nanoblade; pressure controlled cargo delivery; pulsed laser triggered cavitation bubbles; titanium thin film coated micropipette tip; Biomembranes; DNA; Fluorescence; Lasers; Nanoscale devices; Plasmas; Titanium;

fLanguage

English

Publisher

ieee

Conference_Titel

Optical MEMS and Nanophotonics (OMN), 2013 International Conference on

Conference_Location

Kanazawa

ISSN

2160-5033

Print_ISBN

978-1-4799-1512-5

Type

conf

DOI

10.1109/OMN.2013.6659067

Filename

6659067