Generation of large-area tunable uniform electric fields in microfluidic arrays for rapid DNA separation

Author

Huang, L.R. ; Tegenfeldt, J.O. ; Kraeft, J.J. ; Sturm, James C. ; Austin, R.H. ; Cox, E.C.

Author_Institution

Dept. of Electr. Eng., Princeton Univ., NJ, USA

fYear

2001

fDate

2-5 Dec. 2001

Abstract

Control of electric fields over large areas is crucial for the accurate delivery and manipulation of biologically important molecules in microfluidic systems. In this paper we present a novel method for generating tunable uniform electric fields over large microfluidic arrays in two dimensions, and its application to a microfabricated device that separates genomic DNA. The device fractionates large DNA molecules over three orders of magnitude faster than conventional methods.

Keywords

DNA; arrays; bioelectric phenomena; biological techniques; electric fields; electrophoresis; microfluidics; separation; biologically important molecule delivery; biologically important molecule manipulation; electrical current injection method; electrophoresis; fractionation; genomic DNA; large-area tunable uniform electric field generation; microfabricated device; microfluidic arrays; rapid DNA separation; Bioinformatics; Biological materials; Boundary conditions; Current; DNA; Electric potential; Electrodes; Electrokinetics; Genomics; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Electron Devices Meeting, 2001. IEDM '01. Technical Digest. International

Conference_Location

Washington, DC, USA

Print_ISBN

0-7803-7050-3

Type

conf

DOI

10.1109/IEDM.2001.979512

Filename

979512