• DocumentCode
    2166917
  • Title

    Quantitative analysis of living cells by digital holographic microscopy

  • Author

    Reese, Benjamin E. ; Barnett, Robert E. ; Alaoui-Ismaili, Mustapha ; Habibi, Emil ; Mann, Christopher J.

  • Author_Institution
    Dept. of Mech., Univ. of Tennessee, Knoxville, TN
  • fYear
    2009
  • fDate
    18-19 March 2009
  • Firstpage
    1
  • Lastpage
    4
  • Abstract
    Digital holography is applied to obtain noninvasive quantitative phase measurements from living cells. The complex wavefront can be recorded in just a single digital image allowing for critical real-time imaging. The vertical resolution of measurements is determined to be around 3 nanometers.
  • Keywords
    bio-optics; biological techniques; cellular biophysics; holographic interferometry; optical images; optical microscopy; biological microscopy; complex wavefront; digital holographic microscopy; digital image; holographic interference pattern; living cells; noninvasive quantitative phase measurement; quantitative analysis; real-time imaging; vertical resolution; Charge coupled devices; Charge-coupled image sensors; Focusing; Holography; Image reconstruction; Interference; Optical imaging; Optical microscopy; Phase measurement; Phase shifting interferometry;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Biomedical Science & Engineering Conference, 2009. BSEC 2009. First Annual ORNL
  • Conference_Location
    Oak Ridge, TN
  • Print_ISBN
    978-1-4244-3837-2
  • Type

    conf

  • DOI
    10.1109/BSEC.2009.5090485
  • Filename
    5090485