Title :
Isolation and Identification of Endophytic Bacteria Antagonistic to Camellia Oleifera Leaf Blight Base on Informatics
Author :
Li He ; Zhou, Guoying ; Liu, Juang
Author_Institution :
Biotechnol. Core Facilities, Central South Univ. of Forestry & Technol., Changsha, China
Abstract :
Leaf blight is a common Camellia oleifera disease caused by Pestalotiopsis microspora. The damaged young leaves show blight symptom, resulting in yield reduction. This study was to isolate antagonistic bacteria from Camellia oleifera tissue for control of the leaf blight. Antagonistic bacteria was screened from 175 endophytic bacterias by confront culture method. The results of the study showed that the antagonistic effect of YB13 strain was the best with 84.7% inhibitory rate. According to the results of the researching on morphology, cultural characteristics, physiological and biochemical propertices, 16S gene sequence and molecular phylogenetic tree, YB13 was identified as Bacillus subtilis.
Keywords :
biochemistry; biocontrol; bioinformatics; botany; diseases; genetics; microorganisms; Bacillus subtilis; Camellia oleifera leaf blight base; Pestalotiopsis microspora; YB13 strain; antagonistic bacteria; bacteria identification; bacteria isolation; biochemical properties; bioinformatics; cell morphology; confront culture method; culture characteristics; damaged young leaves; disease; endophytic bacteria; gene sequence; molecular phylogenetic tree; physiological properties; yield reduction; Biotechnology; Capacitive sensors; Cultural differences; Forestry; Informatics; Microorganisms; Morphology; Pathogens; Phylogeny; Sequences;
Conference_Titel :
Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on
Conference_Location :
Tianjin
Print_ISBN :
978-1-4244-4132-7
Electronic_ISBN :
978-1-4244-4134-1
DOI :
10.1109/BMEI.2009.5304770
