Isolation and Identification of Endophytic Bacteria Antagonistic to Camellia Oleifera Leaf Blight Base on Informatics

Author

Li He ; Zhou, Guoying ; Liu, Juang

Author_Institution

Biotechnol. Core Facilities, Central South Univ. of Forestry & Technol., Changsha, China

fYear

2009

fDate

17-19 Oct. 2009

Firstpage

1

Lastpage

3

Abstract

Leaf blight is a common Camellia oleifera disease caused by Pestalotiopsis microspora. The damaged young leaves show blight symptom, resulting in yield reduction. This study was to isolate antagonistic bacteria from Camellia oleifera tissue for control of the leaf blight. Antagonistic bacteria was screened from 175 endophytic bacterias by confront culture method. The results of the study showed that the antagonistic effect of YB13 strain was the best with 84.7% inhibitory rate. According to the results of the researching on morphology, cultural characteristics, physiological and biochemical propertices, 16S gene sequence and molecular phylogenetic tree, YB13 was identified as Bacillus subtilis.

Keywords

biochemistry; biocontrol; bioinformatics; botany; diseases; genetics; microorganisms; Bacillus subtilis; Camellia oleifera leaf blight base; Pestalotiopsis microspora; YB13 strain; antagonistic bacteria; bacteria identification; bacteria isolation; biochemical properties; bioinformatics; cell morphology; confront culture method; culture characteristics; damaged young leaves; disease; endophytic bacteria; gene sequence; molecular phylogenetic tree; physiological properties; yield reduction; Biotechnology; Capacitive sensors; Cultural differences; Forestry; Informatics; Microorganisms; Morphology; Pathogens; Phylogeny; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on

Conference_Location

Tianjin

Print_ISBN

978-1-4244-4132-7

Electronic_ISBN

978-1-4244-4134-1

Type

conf

DOI

10.1109/BMEI.2009.5304770

Filename

5304770