Title :
A novel approach to quantitative analysis of electrophoretic gel images of DNA fragments
Author :
Bajla, I. ; Holländer, I. ; Burg, K. ; Fluch, S.
Author_Institution :
Unit of High Performance Image Process., ARC Seibersdorf Research GmbH, Austria
Abstract :
A novel methodology of DNA gel image analysis has been proposed that is based on 2D image processing methods instead of previously used 1D Gaussian deconvolution. The algorithms specifically tailored to band boundary detection and image intensity homogeneity characterization have been developed. The proposed methodology involves a modified type of user interaction.
Keywords :
DNA; biological techniques; deconvolution; edge detection; electrophoresis; gels; image processing; molecular biophysics; 1D Gaussian deconvolution; 2D image processing methods; DNA fragments; DNA molecules separation; biophysical research technique; digitized electrophoretic gels; electrophoretic gel images; image intensity homogeneity characterization; user interaction; Biotechnology; Convolution; DNA; Deconvolution; Electrokinetics; Image analysis; Image processing; Information analysis; Integral equations; Software systems;
Conference_Titel :
Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on
Print_ISBN :
0-7803-7584-X
DOI :
10.1109/ISBI.2002.1029406
