Transfection of Human Embryonic Germ(EG) Cells with Human Cardiac alpha-Actin Promoter Driving pEGFP-N1

Author

Hua, Jinlian ; Yang, Yuai ; Zhuang, Shuzhen ; Dou, Zhongying

Author_Institution

Key Lab. of Mol. Biol. for Agric., Northwest A&F Univ., Yangling, China

fYear

2009

fDate

17-19 Oct. 2009

Firstpage

1

Lastpage

4

Abstract

In order to purify the cardiomyocytes derived stem cells, we constructed an expression vector, alpha -actin-pEGFP-N1 which consists of EGFP gene as reporter markers, and driven by human cardiac alpha-actin promoter, then transfected it into human embryonic germ cells(hEGCs) by Lipofectamine2000. Fluorescent observation and immunocytochemistry staining demonstrated that transfected hEGCs could differentiate into cardiomyocyte like cells and expressed GFP. The results suggest that this system may provide a model for the study of cardiogenesis and differentiation.

Keywords

biochemistry; cardiology; cellular biophysics; fluorescence; Lipofectamine2000; alpha -actin-pEGFP-N1; cardiogenesis; cardiomyocytes; differentiation; expression vector; fluorescence; human cardiac alpha-actin promoter; human embryonic germ cells; immunocytochemistry staining; stem cells; transfaction; Cardiology; Cells (biology); Embryo; Heart; Humans; In vitro; Mice; Muscles; Pathogens; Stem cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on

Conference_Location

Tianjin

Print_ISBN

978-1-4244-4132-7

Electronic_ISBN

978-1-4244-4134-1

Type

conf

DOI

10.1109/BMEI.2009.5305494

Filename

5305494