Generators and applicators for nanosecond pulsed electric field

This paper describes nanosecond electric pulse generator and applicator for biological experiment. For the generator, an optoelectronic switching embedded in a frozen wave generator is obtained in the linear regime. In this way, square electrical pulses with adjustable duration and monocycle nanosecond pulses with balanced or unbalanced positive and negative components are obtained. In biological experiments, these nsPEF generators are frequently coupled with a standard electroporation cuvette, 50-Ω matched, containing the biological sample. A numerical and experimental characterization of a 50-Ω applicator is presented.