Digital autoradiography imaging using direct irradiation of a CCD between 278-309 K

Using direct irradiation of a CCD for beta autoradiography is principally limited by variations in cumulative pixel dark current across the device modulated by small changes in temperature. We have used a modified fixed pattern noise correction method to address this problem at room temperature, and tested the method using ¹⁴C microscales and labeled mouse tissue samples. An evaluation is included of the minimum activity levels which can be detected between 278 K and 309 K, determining the quantitative and qualitative imaging capabilities and improvements of such an approach for imaging distributed sources (such as the microscales) at various levels of activity.