Title :
Sequence Analysis of rDNA ITS of Clinical Fusarium Species
Author :
Li He ; Zhou, Guoying ; Liu, Junang
Author_Institution :
Biotechnol. Core Facilities, Central South Univ. of Forestry & Technol., Changsha, China
Abstract :
The study on molecular structure of Fusarium proliferatum clinically isolated can provide the basis for accurate diagnosis and treatment of F. proliferatum infection. The rDNA ITS gene of 6 strains that had been identified as Fusarium proliferatum based on morphological were cloned and sequenced. The molecule phylogenetic tree was constructed based on rDNA ITS sequences, and the 6 genus were divided into two groups. Group I was F.proliferatum, including strains of CSUFT201, CSUFT204, CSUFT205. Group II was F. moniliforme, including CSUFT202, CSUFT203, CSUFT206. The results of this study showed that 3 strains of F. moniliforme were confirmed out of the 6 strains with the molecular methods.
Keywords :
DNA; genetics; microorganisms; molecular biophysics; CSUFT201; CSUFT202; CSUFT203; CSUFT204; CSUFT205; CSUFT206; F. moniliforme; Fusarium proliferatum infection; molecular structure; molecule phylogenetic tree; rDNA ITS gene; sequence analysis; Biological materials; Biotechnology; Capacitive sensors; Cloning; DNA; Educational institutions; Forestry; Isolation technology; Phylogeny; Sequences;
Conference_Titel :
Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on
Conference_Location :
Tianjin
Print_ISBN :
978-1-4244-4132-7
Electronic_ISBN :
978-1-4244-4134-1
DOI :
10.1109/BMEI.2009.5305743
