Environmental chamber for microscopic observation

An environmental chamber will be constructed to monitor and control factors that mimic physiologic conditions while studying live cells and tissue cultures. The chamber to be installed on different varieties of inverted microscopes and has to enclose the space around the stage for proper measurement and control. The system will monitor temperature, CO₂/pH, as well as humidity in a closed feedback loop to keep the parameters nearly constant during microscopy. The control of the parameters must be effective and be constant throughout the chamber, which means good circulation and distribution of heat, humidity, and CO₂. For the control and monitoring, a microprocessor based on an Arduino board was being studied to take measurements as well as initiate the feedback system to keep parameters constant. The design of the environmental chamber has been finalized and construction will begin shortly after the ordered materials arrive, and the computer interface and control will be done for the measurement of parameters and coupled with the sensors to begin testing.