Title :
Development of a Fourier transform infrared imaging spectroscopic assay for screening estrogenic contaminants
Author :
Johnson, C.M. ; Pleshko, Nancy ; Achary, M. ; Suri, R.P.
Author_Institution :
Dept. of Civil & Environ. Eng., Univ. Philadelphia, Philadelphia, PA, USA
Abstract :
There is a need for a rapid bioassay to assess estrogen pollutants in water. This study evaluates the use of Fourier Transform Infrared Imaging Spectroscopy (FT-IRIS) as a screening tool for estrogenic effects on estrogen receptor positive breast cancer cells, MCF-7 cells. FT-IRIS acquired spectra revealed a dose response in the carbohydrate and or serine/tyrosine region in single MCF-7 cells after a 48-hour incubation with 17β-estradiol. This response was found to be comparable to that of a validated E-Screen assay, which utilizes the same cells and is 6 days in duration. These data provide support for further validation of this spectroscopic assay.
Keywords :
Fourier transform spectra; biomedical optical imaging; cancer; cellular biophysics; infrared spectra; spectrochemical analysis; 17β-estradiol; FT-IRIS acquired spectra; Fourier transform infrared imaging spectroscopic assay; MCF-7 cells; carbohydrate; dose response; estrogen pollutants; estrogen receptor positive breast cancer cells; estrogenic contaminants; estrogenic effects; incubation; screening tool; serine-tyrosine region; time 48 hour; water; Biochemistry; Educational institutions; Fourier transforms; Imaging; Lipidomics; Spectroscopy; USA Councils;
Conference_Titel :
Bioengineering Conference (NEBEC), 2012 38th Annual Northeast
Conference_Location :
Philadelphia, PA
Print_ISBN :
978-1-4673-1141-0
DOI :
10.1109/NEBC.2012.6206960
