Nanophase magnesium for orthopedic applications

Magnesium has gained interest as a biomaterial for orthopedic applications because of its biocompatibility, biodegradability, and positive effect on bone formation. Likewise, studies have shown nanophase material increase osteoblast (bone-forming cell) function compared to conventional materials, but the two have not been studied together. The purpose of this study was to determine if altering magnesium surface features into the nanometer scale promotes greater osteoblast functions. Nanorough magnesium surfaces were created by a novel treatment with sodium hydroxide at 1N, 5N, and 10N concentrations for 10, 20, and 30 minutes. Material characterization by scanning electron microscopy showed increased roughness on all treated samples compared to the control magnesium. Contact angle measurements indicated greater hydrophilicity on treated magnesium and no significant effect of ultraviolet sterilization on the surface energy of the material. Osteoblasts were seeded onto treated and untreated surfaces and adhesion at 4hrs were assessed through the MTT assay. Results indicated increased osteoblast adhesion on nano-treated samples compared to untreated samples. These findings support previous studies indicating the promise of magnesium as a biomaterial for orthopedic applications and suggest further experiments examining the long-term effects of nanophase magnesium on osteoblast proliferation and function.