Title :
A microfluidic array with micro sieves for cell cytotoxicity screening
Author :
Wang, Zhanhui ; Kim, Min-Cheol ; Thorsen, Todd
Author_Institution :
Key Lab. for Biomed. Inf. & Health Eng., Chinese Acad. of Sci., Shenzhen
Abstract :
A microfluidic array with micro cell sieves was designed to fabricate multiple cells array for high-throughput cell cytotoxicity screening. Micro channels in both directions (column and row) were individually addressable by using a set of elastomeric monolithic array valves, enabling parallel loading of various cell lines in one direction and introducing of different toxins in the other direction. Several micro cell sieves were designed in each chamber. Cell number and distribution in chambers can be conveniently controlled by the adjustment of cell sieve number, distribution and size. Fluid transport property and cell loading in chamber were simulated and verified experimentally. The capability of the microfluidic array was demonstrated for seeding multiple mammalian cell lines in chambers and observing the cell viabilities after cells exposed to different toxins with a fluorescence microscope.
Keywords :
bioMEMS; biomedical measurement; cellular biophysics; fluorescence; microchannel flow; microvalves; toxicology; cell cytotoxicity screening; cell loading; cell sieve distribution; cell sieve size; elastomeric monolithic array valves; fluid transport property; fluorescence microscope; microcell sieves; microchannels; microfluidic array; multiple cells array; multiple mammalian cell lines; Biomedical engineering; Charge carrier processes; Drugs; Holographic optical components; Mechanical engineering; Microchannel; Microfluidics; Nonhomogeneous media; Size control; Valves;
Conference_Titel :
Information Technology and Applications in Biomedicine, 2008. ITAB 2008. International Conference on
Conference_Location :
Shenzhen
Print_ISBN :
978-1-4244-2254-8
Electronic_ISBN :
978-1-4244-2255-5
DOI :
10.1109/ITAB.2008.4570519
