Optimization of laser-induced fluorescence detection system for microfluidic chip

Author

Shi, Pan ; Hu, Xiaoming ; Li, Qin ; Deng, Yulin

Author_Institution

Dept. of Biomed. Eng., Beijing Inst. of Technol., Beijing

fYear

2008

fDate

30-31 May 2008

Firstpage

207

Lastpage

210

Abstract

Laser-induced fluorescence (LIF) detection is widely utilized in microfluidic chip due to its high sensitivity and selectivity. In this paper, a LIF detection system for the microscope chip based on a 473 nm laser diode is described. To improve the performance of the detection system, the confocal configuration is adopted in our experiment. Some factors that are important to the signal-to-noise ratio (SNR) of the confocal system, such as the choice of microscope objective, effect of excitation source, the size of pinhole and the performance of the PMT detection units are presented and explained in this paper.

Keywords

bioMEMS; biomedical measurement; biomedical optical imaging; fluorescence; lab-on-a-chip; laser applications in medicine; measurement by laser beam; microfluidics; optical microscopy; photomultipliers; semiconductor lasers; LIF detection; PMT detection units; SNR; confocal configuration; excitation source; laser diode; laser-induced fluorescence detection system; microfluidic chip; microscope chip; pinhole size; signal-to-noise ratio; wavelength 473 nm; Biomedical optical imaging; Diode lasers; Fluorescence; Laser beams; Laser excitation; Microfluidics; Microscopy; Optical filters; Optical sensors; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Technology and Applications in Biomedicine, 2008. ITAB 2008. International Conference on

Conference_Location

Shenzhen

Print_ISBN

978-1-4244-2254-8

Electronic_ISBN

978-1-4244-2255-5

Type

conf

DOI

10.1109/ITAB.2008.4570634

Filename

4570634