Title :
Applications of Protein-Based Capacitive Biosensors for the Detection of Heavy-Metal Ions
Author :
Mortari, Alessia ; Brown, Nigel L. ; Geczy, Carolyn ; Coster, Hans G L ; Valenzuela, Stella M. ; Martin, Donald ; Csoregi, Elisabeth
Author_Institution :
Dept. of Anal. Chem., Lund Univ.
Abstract :
Three different methods of using protein-based capacitive biosensors for the detection of heavy-metal ions are presented. The metal-binding proteins SmtA, S100A12, MerP and four modified MerPs were immobilised as the bio-recognition element on self-assembled monolayer-modified gold electrodes. Capacitance was measured using potential square step or electrical impedance spectroscopy. The protein-metal interaction generated changes in capacitance mainly due to a protein conformational change.
Keywords :
biosensors; electrodes; gold; monolayers; proteins; bio-recognition element; biomedical application; electrical impedance spectroscopy; electrochemical capacitance; environmental application; heavy-metal ion detection; metal-binding proteins; protein-based capacitive biosensors; self-assembled monolayer-modified gold electrodes; Australia; Biochemical analysis; Biomedical measurements; Biosensors; Capacitance measurement; Chemical technology; Chemistry; Electrodes; Pathology; Protein engineering;
Conference_Titel :
Nanoscience and Nanotechnology, 2006. ICONN '06. International Conference on
Conference_Location :
Brisbane, Qld.
Print_ISBN :
1-4244-0452-5
Electronic_ISBN :
1-4244-0452-5
DOI :
10.1109/ICONN.2006.340595
