Title :
Organosilica Particles for DNA Screening Applications
Author :
Corrie, Simon ; Lawrie, Gwen ; Battersby, Bronwyn ; Trau, Matt
Author_Institution :
Sch. of Molecular & Microbial Sci., Queensland Univ., St. Lucia, Qld.
Abstract :
Particle-based libraries for chemical screening are of interest for robust biochemical assays and molecular diagnostic applications. Silica particles are chemically stable and possess two separate chemical modification pathways that facilitate the attachment of fluorescent dyes for encoding purposes and specific oligonucleotides for hybridization assays. Characterization of surface modifications of the particles are presented and quantitation of oligonucleotide probes on the particle surface is described. The application of the particles in biological screening experiments is presented by means of a single base mismatch detection assay.
Keywords :
DNA; dyes; molecular biophysics; DNA screening; biological screening; chemical modification pathways; fluorescent dyes; hybridization assays; mismatch detection assay; oligonucleotides; organosilica particles; surface modifications; Chemicals; DNA; Fluorescence; Libraries; Probes; Rough surfaces; Scanning electron microscopy; Stability; Surface morphology; Surface roughness;
Conference_Titel :
Nanoscience and Nanotechnology, 2006. ICONN '06. International Conference on
Conference_Location :
Brisbane, Qld.
Print_ISBN :
1-4244-0453-3
Electronic_ISBN :
1-4244-0453-3
DOI :
10.1109/ICONN.2006.340599
