Title :
Visualization of dose effects on cultivated cells in photodynamic therapy by a laser speckle microscopy
Author :
Hirakawa, Yasuyuki ; Fukunaga, Yukihiro ; Miyoshi, Norio
Author_Institution :
Dept. of Electr. & Electron. Eng., Kurume Nat. Coll. of Technol., Fukuoka, Japan
Abstract :
Visualization of regent-dose effects on human prostate cancer cells in photodynamic therapy (PDT) was tried by microscopic laser speckle observations. The used regent was 5-aminolevulinic acid (5-ALA), which induces protoporphyrin-IX to give photodynamic damages to cells with 635 nm laser illumination. By analyzing frequency spectra of laser speckle fluctuation (LSF) observed on cells in PDT, it suggested that LSFs at cellular membranes and inside cells indicate different behavior each other.
Keywords :
biomembranes; cancer; cellular effects of radiation; laser applications in medicine; photodynamic therapy; speckle; 5-aminolevulinic acid; cellular membrane; cultivated cell; dose effect visualization; human prostate cancer cell; laser illumination; laser speckle microscopy; photodynamic therapy; protoporphyrin-IX; regent-dose effect; wavelength 635 nm; Cells (biology); Fluctuations; Lasers; Microscopy; Speckle; Time frequency analysis; Visualization;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
