Title :
Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection
Author :
Pokhriyal, Anusha ; Lu, Meng ; Chaudhery, Vikram ; Huang, Cheng-Sheng ; Schulz, Stephen ; Cunningham, Brian T.
Author_Institution :
Dept. of Phys., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA
Abstract :
A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500x for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330x SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140x lower limit of detection compared to a conventional glass substrate.
Keywords :
fluorescence; nanolithography; photonic crystals; quartz; deposited fluorophore-tagged protein spots; dose-response characterization; nanoimprint lithography; photonic crystal enhanced fluorescence; quartz substrate; signal enhancement factor; Fluorescence; Glass; Optical surface waves; Photonic crystals; Signal to noise ratio; Surface treatment; Surface waves;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
