Numerical analysis of FRAP experiments for DNA replication and repair

Author

Cinquemani, Eugenio ; Roukos, Vassilis ; Lygerou, Zoi ; Lygeros, John

Author_Institution

Autom. Control Lab., ETH Zurich, Zurich, Switzerland

fYear

2008

fDate

9-11 Dec. 2008

Firstpage

155

Lastpage

160

Abstract

In this paper we describe a stochastic model for particle diffusion and binding within cell nuclei, and a numerical approximation of it that is well suited for numerical simulation and investigation of Fluorescence Recovery After Photobleaching (FRAP) experiments. An overview of the theory and of the implementation of the method is given. Simulation results are reported and are used to analyze data from real photobleaching experiments, mainly at a qualitative level.

Keywords

DNA; biocomputing; biology computing; numerical analysis; DNA replication; cell nuclei; deoxyribonucleic acid; fluorescence recovery; numerical analysis; numerical approximation; numerical simulation; particle diffusion; photobleaching experiments; stochastic model; Bioinformatics; Biological system modeling; DNA; Data analysis; Fluorescence; Genomics; Numerical analysis; Numerical simulation; Photobleaching; Proteins; Biological systems; Monte Carlo methods; Numerical simulation; Parameter identification; Stochastic approximation;

fLanguage

English

Publisher

ieee

Conference_Titel

Decision and Control, 2008. CDC 2008. 47th IEEE Conference on

Conference_Location

Cancun

ISSN

0191-2216

Print_ISBN

978-1-4244-3123-6

Electronic_ISBN

0191-2216

Type

conf

DOI

10.1109/CDC.2008.4738718

Filename

4738718