Title :
The use of hemizona assay as a model for sperm-oocyte interaction in the evaluation of the optimal sperm preparation technique
Author :
Paz, Gerardo ; Amit, A.
Author_Institution :
Inst. for the Study of Fertility, Tel Aviv Univ.
Abstract :
This study was designed to evaluate the effect of different sperm preparation methods on sperm binding capacity by using the hemizona assay (HZA). Seventy men were included in the study. Four different techniques (swim-up, TEST-yolk buffer [TYB], Percoll and pentoxifylline [PTX]) were used for preparation of sperm suspensions. The routine swim-up preparation was used as the reference method. Only TYB and PTX exhibited an overall statistically significant improvement in sperm binding capacity in comparison with the swim-up preparation method (P=0.01 and P=0.001, respectively)
Keywords :
biological specimen preparation; cellular biophysics; Percoll; TEST-yolk buffer; hemizona assay; infertility clinic; optimal sperm preparation technique evaluation; pentoxifylline; sperm binding capacity; sperm suspensions; sperm-oocyte interaction model; swim-up; Hospitals; Humans; In vitro fertilization; Indium; Laboratories; Micromanipulators; Temperature; Testing;
Conference_Titel :
Engineering in Medicine and Biology Society, 1995 and 14th Conference of the Biomedical Engineering Society of India. An International Meeting, Proceedings of the First Regional Conference., IEEE
Conference_Location :
New Delhi
Print_ISBN :
0-7803-2711-X
DOI :
10.1109/RCEMBS.1995.511696
