Pinpoint injection of micro tools using dielectrophoresis and hydrophobic surface for minimally invasive separation of microbe

We proposed a pinpoint injection method of micro tools (MTs) at the desired location in the micro chamber, which is filled with liquid. We demonstrated a new method to install the micro tools inside the micro chamber. We developed a micro tool holding chip to install the micro tools. The micro tools were aligned in the micro groove autonomously by the dielectrophoresis and liquid bridge force of the hydrophobic surface. Then, MTs were injected in the micro chamber, and were manipulated successfully by the laser scanning micromanipulator to separate the target microbe. It is the first demonstration of the pinpoint injection of MTs and separation by the indirect micromanipulation.