DNA kinetics in microfabricated devices

Author

Yick Chuen Chan ; Ma, R.M.S. ; Carles, M. ; Sucher, N.J. ; Man Wong ; Zohar, Y.

Author_Institution

Dept. of Mech. Eng., Hong Kong Univ. of Sci. & Technol., Kowloon, China

fYear

2002

fDate

24-24 Jan. 2002

Firstpage

60

Lastpage

63

Abstract

The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.

Keywords

DNA; biochemistry; biodiffusion; biological techniques; capillarity; electrochemical analysis; electrophoresis; molecular biophysics; 14bp-DNA fragment mobility; DNA kinetics; DNA plug motion; agarose gel; branching system; channel cross-sectional area; diffusion coefficient; downstream branch; equivalent length; hydroxyethylcellulose polymer solution; kinematic relationship; micro-capillary electrophoresis; microfabricated devices; miter bend; separation sieving matrices; Bonding; DNA; Electrodes; Electrokinetics; Glass; Kinetic theory; Packaging; Plugs; Polymer gels; Sputter etching;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2002. The Fifteenth IEEE International Conference on

Conference_Location

Las Vegas, NV, USA

ISSN

1084-6999

Print_ISBN

0-7803-7185-2

Type

conf

DOI

10.1109/MEMSYS.2002.984083

Filename

984083